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Image Search Results
Journal: Scientific Reports
Article Title: Effect of Cucumis sativus on Dysfunctional 3T3-L1 Adipocytes
doi: 10.1038/s41598-019-49458-6
Figure Lengend Snippet: Superoxide induction by IL-1β in 3T3-L1 adipocytes. Mature adipocytes (day 8) were cultured for 24, 48, 60, or 72 h, either alone, with IL-1β, and/or insulin 1 μM. Microphotographs were taken in a spectrofluorometer under a 20X objective at 24 ( A ), 48 ( B ), 60 ( C ), and 72 h ( D ). The percent of DHE expression was determined ( E ) with the software MetaMorph v.6.1. Results are expressed as mean ± SD, *P ≤ 0.05 with respect to cells treated with insulin 1 μM and were analyzed by ANOVA and the post-hoc Tukey-Kramer test, n = 6.
Article Snippet: The percent of
Techniques: Cell Culture, Expressing, Software
Journal: Methods in molecular biology (Clifton, N.J.)
Article Title: Analysis of Signaling Pathways in Zebrafish Development by Microinjection
doi: 10.1007/978-1-59745-202-1_6
Figure Lengend Snippet: Quantitation of fertilization-induced changes in calcium green fluorescence. Oocytes were injected with calcium green dextran and GST, then images were recorded after a 10-min recovery period. Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using Metamorph 6.1 software. The top panel represents global fluorescence measured from a cross-section of the entire zygote. The middle panel represent fluorescence measured within a circular region in the center of the zygote (central cytoplasm) comprising approximately 25% of the total cross-sectional area. The bottom panel represents fluorescence measured within an arc traced over the cortex (cortical cytoplasm) as near as possible to the micropyle. This arc comprised approximately 20% of the perimeter of the zygote.
Article Snippet: Fertilization was initiated by addition of a mixture of sperm and water at time 0 and images were recorded every 15 s. Fluorescence was quantitated by pixel intensity quantitation using
Techniques: Quantitation Assay, Fluorescence, Injection, Software